Alkaline hydrolysis of poly(γ-glutamic acid) produced by microorganism

The structure of ribonucleic acid. I. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis.

Synthesis and enzymatic hydrolysis of glutamic acid polypeptides.

Synthetic lysine polypeptides of high molecular weight (1) have been shown to reduce virus infectivity (2, 3), inhibit bacterial growth and respiration (4), agglutinate virus particles, bacterial cells, and erythrocytes (4-6), and to prolong blood clotting time (7). It has been suggested that most of these phenomena are associated with the formation of many ionic bonds between the positively ch...

Biosynthesis and Hydrolysis of Poly ( 7 - glutamic acid ) from BaciUizs

Poly(7-glutamic a id) (PGA) production ill Bacillas suhtitis IF03335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA wEs produced. On the other hand, when glucose was added to the glutamic acid medium, a by-preduct was produced, which seemed to be a polysaccharide. Moreoyer, the mode...

Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells

The effect of poly-γ-glutamic acid (γPGA), which is produced by Bacillus sp., on the production of recombinant erythropoietin (rEPO) by Chinese hamster ovary (CHO) cells in suspension culture was evaluated. The growth, viability, and productivity of recombinant CHO cells were investigated in a chemically defined medium with 50 and 500 kD γPGAs at 0.075% or with Pluronic F68. Cell growth with th...

Hydrolysis of S-substituted monoesters of phosphorothioic acid by alkaline phosphatase from Escherichia coli.

Alkaline phosphatase from Escherichia coli catalyzes the hydrolysis of S-substituted monoesters of phosphorothioic acid of the type RSP03Naz (R = -CH2CH2NH2,-CH2CHzNHCOCH3,-CH,COOH, or -CH2CHzCOOCzH~), at the S-P bond, to yield orthophosphate and the corresponding thioalcohols. The rate of enzymic hydrolysis of cysteamine S-phosphate was measured at different pH values and substrate concentrati...